Agilent 1260 Infinity HPLC System

Specifications:
G1311C Quat Pump VL
Hydraulic system Dual piston in series pump with servo-controlled variable stroke drive, power transmission by gears and ball screws, floating pistons
Settable flow range Set points 0.001 – 10 mL/min, in 0.001 mL/min increments
Flow range 0.2 – 10.0 mL/min
Flow precision ≤0.07 % RSD, or ≤ 0.02 min SD whatever is greater, based on retention time at constant room temperature
Flow accuracy ± 1 % or 10 µL/min whatever is greater, pumping degassed H2O at 10 MPa (100 bar)
Pressure pulsation < 2 % amplitude (typically < 1.0 %), or < 0.3 MPa (3 bar, 44 psi), whatever is greater, at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar, 145 psi)
Compressibility compensation User-selectable, based on mobile phase compressibility
Recommended pH range 1.0 – 12.5 , solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient formation Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve
Delay volume 600 – 900 µL, dependent on back pressure; measured with water at 1 mL/min (water/caffeine tracer)
Integrated degassing unit Number of channels: 4 Internal volume per channel: 1.5 mL

G1316A TCC Thermostated Column Compartment
Temperature Range 10° below ambient to 80° C
Temperature Stability ± 0.15°C
Temperature Accuracy ± 0.8°C
Column Capacity Three 30cm columns
Warm Up/Cool Down :
5 mins from ambient to 40°C
10 minutes from 40 – 20 °C
Dead Volume :
3 µl left heat exchanger
6 µl right heat exchanger

G1329B ALS Auto Sampler
Pressure Operating range 0 – 60 MPa (0 – 600 bar, 0 – 8850 psi)
Injection range 0.1 – 100 µL in 0.1 µL increments (recommended 1 µL increments) Up to 1500 µL with multiple draw (hardware modification required)
Replicate injections 1 – 99 from one vial
Precision Typically < 0.25 % RSD of peak areas from < 5 µL to 100 µL Typically < 1 % RSD of peak areas from 1 µL to 5 µL
Minimum sample volume 1 µL from 5 µL sample in 100 µL microvial, or 1 µL from 10 µL sample in 300 µL microvial
Carryover Typically < 0.1%, < 0.05% with external needle cleaning
Sample viscosity range 0.2 – 50 cp
Sample capacity :
100 × 2 mL vials in 1 tray
40 × 2 mL vials in ½ tray
15 × 6 mL vials in ½ tray (Agilent vials only)
Injection cycle time 50 s for draw speed 200 µL/min, ejection speed 200 µL/min, injection volume 5 µL
Metering device Metering pump in high pressure flow path
G1330B Thermostat
Temperature range Settable from 4 °C to 40 °C in 1 ° increments
emperature accuracy at ambient temperatures < 25 °C and humidity < 50 % 3 °C to 8 °C at a setpoint of 4 °C*
Temperature accuracy at ambient temperatures 3 °C to 9 °C at a setpoint of 4 °C*
G1364C y Analytical-scale Fraction Collector
Fraction vessel capacities and trays :
4 x well-plates full tray (MTP)*
2 x well-plates std. tray + 10 funnels with external containers* (+ 1 half tray)
2 × well-plates std. tray (MTP) + 10 × 2 ml vials* (+ 1 half tray)
100 x 2 ml in std. tray (+ 1 half tray)*
3 x 40 x 2 ml in half tray*
3 x 40 funnels in half tray
3 x 15 x 6 ml in half tray*

Full tray with 40 test tubes (30 mm OD, max. height 48 mm, ~20 ml vol.)
Full tray with 60 test tubes (25 mm OD, max. height 48 mm)
Full tray with 126 test tubes (16 mm OD, max. height 48 mm)
Full tray with 215 test tubes (12 mm OD, max. height 48 mm) Installed trays are automatically detected and identified.
Installed plates and vials can be detected when operating in the needle into location mode
* max. height can be extended by using the short needle assembly
Maximum tube / plate height 48 mm with long needle assembly G1367-87200 75 mm with short needle assembly G1364-87202
Maximum tube volume ca. 20 ml with 48 mm test tubes, ca. 30 ml with 75 mm test tubes or unlimited, if funnels are used with external containers.
Maximum flow rate 10 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve). The analytical scale fraction collector can be modified for flow rates > 10 ml/min.
Delay volumes [μl] Fraction collector inlet to diverter valve: ~50 (typical, depends on the length of the tubing) Diverter valve: ~15 Diverter valve to needle: ~10 Needle: ~4
Delay calibration sensor Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode
Diverter valve 3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar

G1365D MWD Detector
Detection type 1024-element photodiode array
Light source Deuterium and tungsten lamps
Number of signals 8
Maximum sampling rate 20 Hz
Wavelength range 190 – 950 nm
Short term noise < ± 0.7·10-5 AU at 254 and 750 nm
Drift < ± 0.7 x 10-5 AU at 254/4 nm and at 750 nm, TC 2 sec.
Linear absorbance range > 2 AU (upper limit)
Wavelength accuracy ± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter
Wavelength bunching 1 – 400 nm Programmable in steps of 1 nm
Slit width 1, 2, 4 , 8, 16 nm Programmable slit
Diode width < 1 nm
Flow cells Standard:
13-µL volume, 10 mm-cell path length, 120 bar (1740 psi) pressure maximum
Semi-micro
5-µL volume, 6-mm cell path length, 120 bar (1740 psi) pressure maximum
Micro
2-µL, volume, 3-mm cell path length, 120 bar (1740 psi) pressure maximum
Semi-nano
500-nanoliter volume, 10-mm cell path length, 50 bar (725 psi) pressure maximum
Nano
80-nanoliter volume, 6-mm cell path length, 50 bar (725 psi) pressure maximum
High Pressure (for SFC)
1.7-µL volume, 6-mm cell path length, 400 bar (5802 psi) pressure maximum
Preparative
3 mm 3-mm cell path length, 120 bar (1740 psi) pressure maximum
Preparative
3 mm 0.3-mm cell path lenght, 20 bar (291 psi) pressure maximum, quartz

Preparative
0.06 mm 0.06-mm cell path length, 20 bar (291 psi) pressure maximum, quartz
General
Dimensions 25x14x17 per stack (2 stacks)
Weight 100 lbs
Power 100-120/220-240 V, 50/60 Hz