BD Biosciences FacsCanto II – 3 Laser w/ Fluidics Cart Flow Cytometer

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient,
easy-to-use yet powerful six-color benchtop analyzer.
It incorporates many technology advances first developed in the BD LSR II expandable analyzer
and the BD FACSAria cell sorter. It supports a broad range of research applications,
being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

The BD FACSCanto system combines a patented optical design for enhanced signal retention
on six fluorescent and two scatter parameters, digital electronics for processing
up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%.
Your total benefit consists of the best fluorescence sensitivity to resolve dim events,
cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science.
BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

Includes:
Computer w/ FacsDiva Software

Fluids Cart
2 laser, Blue(488) and Red (633)
Laser
6 PMTs unit and side scatter

All Necessary Cables
Manuals

Optics Specifications:
Lasers
Air-cooled:
488-nm solid state, 20-mW laser output
633-nm HeNe, 17-mW laser output
Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
Optical Alignment Procedure: Fixed, no operator alignment required
Flow Cell: 180 x 430-μm rectangular quartz flow cell
Collection Optics: Optical-gel coupled 1.2 NA lens
FSC Resolution: 0 μm
SSC Resolution: 5 μm
Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
FSC Detector: Photodiode with 488/10 BP
SSC Detector: PMT with 488/10 BP
Fluorescence Detectors: 6 PMTs in 4-2 standard configuration
Blue Laser Dyes
FITC, PE, PerCP or PerCP-Cy™5.5,
PE-Cy7 (525, 575, 678 or 695, 785 nm)
Red Laser Dyes: APC, APC-Cy7 (660, 785 nm)
Detector Bands
Blue Laser: 530/30; 585/42; >670; 780/60 nm
Red Laser: 660/20; 780/60 nm
Fluorescence Threshold Sensitivities: FITC