MJ Research DNA Engine Fluorescence Detector

An easy-to-use and sensitive machine for measuring continuous fluorescence, particularly in real-time quantitative PCR (qPCR) applications. The detector itself is available as a complete system (including a desktop computer, monitor, and DNA engine thermal cycler) or as an upgrade to an existing thermal cycler. This system has an excitation range of 450-495 nm and a detection range of 515-545 nm. It has a heated lid which eliminates the need for oil on top of samples and samples can be run in either 96-well plates or strip tubes. It has a temperature range of 0 to 150°C with an accuracy of ±0.3°C and a relatively quick ramping speed: up to 3°C per second. A temperature gradient feature is available that allows the user to easily optimize the annealing temperature of the PCR reaction by running each column of wells at a separate temperature. A gradient calculator is built into the software which makes it very easy to calculate the well temperature across all 12 columns. The Opticon is compatible with many different chemistries including SYBR® Green I, Molecular Beacons, TaqMan® Probes, Scorpion® Probes, and the Amplifluor® System