Molecular Devices Spectramax M5 Microplate Reader

Specifications:
Read modes Absorbance
Fluorescence Intensity
Luminescence
Time-Resolved Fluorescence
Fluorescence Polarization
Robotic-compatible Yes
Plate formats 6, 12, 24, 48, 96, 384 wells
Light source Xenon Flash Lamp (1 joule/flash)
Detectors 2 photomultiplier tubes (PMT)
Shaker time 0 to 999 seconds
Temp. control 2°C above ambient to 60°C
Temp. uniformity < 1°C at 37°C set point
Temp. accuracy ±1°C at 37°C set point
Readings Endpoint, Kinetic Spectral Scanning
Standard Read Times (minutes: seconds)* 96 wells 384 wells
Absorbance 0:18 0:49

Fluorescence Intensity 0:17 0:48
Luminescence 2:00 7:00
Absorbance Photometric Performance
Reading Capabilities Cuvette or microplate
Wavelength range 200–1000 nm
Wavelength selection Monochromator, tunable in 1.0 nm increments
Wavelength bandwidth ≤ 4.0 nm
Wavelength accuracy ±2.0 nm
Wavelength repeatability ±0.2 nm
Photometric range 0–4.0 OD
Photometric resolution 0.001 OD
Photometric accuracy (microplate) < ±0.006 OD ±1.0%, 0–2 OD

(cuvette) < ±0.005 OD ±1.0%, 0–2 OD


Photometric precision < ±0.003 OD ±1.0%, 0–2 OD
Stray light < 0.05% @ 230 nm
Fluorescence Intensity Performance Top or bottom
Wavelength range 250–850 nm
Wavelength selection Monochromators, tunable in 1.0 nm increments
Bandwidth (EX, EM) 9 nm, 15 nm
Optimized sensitivity ≤ 1 pM fluorescein in 96 wells,
≤ 1.5pM in 384 wells
Guaranteed sensitivity < 5 pM fluorescein in 96 wells or cuvette,
< 20 pM in 384 wells
Luminescence Performance Top or Bottom
Wavelength selection Choice of simultaneous detection of all wavelengths or selection via monochromator, tunable in 1.0 nm increments
Wavelength range 250–850 nm
Optimized sensitivity ≤ 43pM ATP in 96 wells
Guaranteed sensitivity ≤ 75pM ATP in 96 wells
Dynamic range > 6 decades
Cross-talk < 0.3% in white 96- and 384-well Microplates
Time Resolved Fluorecesnce Performance Top or Bottom of a Microplate
Wavelength selection Monochromators, tunable in 1.0 nm increments
Bandwidth (EX, EM) 9 nm, 15 nm
Precision data collection 1–100 flashes, delay of 0–600 µsec. before read, integration time selectable between 50–1500 µsec.
Optimized sensitivity ≤ 10 fM europium in 96
Guaranteed sensitivity ≤ 100 fM europium in 96 or 384 wells
Fluorescence Polarization Performance
Wavelength range 300–750 nm
Wavelength selection Monochromators, tunable in 1.0 nm increments
Bandwidth (EX, EM) 9 nm, 15 nm
Optimized Precision ≤ 3.5 mP standard deviation at 1 nM fluorescein in 96 wells
Guaranteed Precision < 5 mP standard deviation at 1 nM fluorescein in 96 wells
Dimensions 22x16x9”
Weight 36 lbs
Power < 420 watts, 100–240 VAC, 3.5 A, 50/60Hz