Thermo Scientific Vanquish UHPLC Sytem

Autosampler

Purpose: Introduces the sample into the mobile phase stream automatically.
Picks up sample from vials, plates, or tubes
Injects with high precision (often nL to µL volume range

2.Pump Module
Purpose: Delivers the mobile phase (solvent) through the system at high pressure.
Binary pump: Mixes two solvents at high pressure. Ideal for fast gradients.

Quaternary pump: Mixes up to four solvents at low pressure. More flexible, but slower gradients.
Controls flow rate (e.g., 0.001–5.000 mL/min)
Enables gradient elution (changing solvent composition over time

3.Detector Module (x2)
Purpose: Detects and measures analytes as they elute from the column.
Diode Array Detector (DAD/UV-Vis) – most common; detects UV-absorbing compounds
Fluorescence Detector – for fluorescent compounds
Charged Aerosol Detector (CAD) – universal detection for non-UV compounds
Refractive Index Detector – for sugars, polymers
MS (Mass Spectrometer) – advanced, highly sensitive detection

Thermo Fisher’s Vanquish platform includes several variants tailored to different analytical needs—from routine HPLC to ultra-high-throughput and low-flow LC–MS workflows: