BD BioScience Accuri C6 Flow Cytometer

Includes:
Recent PM
Computer loaded with Accuri Software
BD CSampler Accessory
(2) Laser Configuration

Specifications:
Optics
Laser Excitation
488 nm (rated at 20,000-h life)
640 nm (rated at 20,000-h life)
Laser Profile: 10 x 75 μm
Light Scatter Detection
Forward (0°, ±13°)
Side (90°, ±13°)
Emission Detection
4 colors, user-changeable optical filters
Standard set installed:
FL1 533/30 nm (eg, FITC/GFP)
FL2 585/40 nm (eg, PE/PI)
FL3 > 670 nm (eg, PerCP,PerCP-Cy5.5, PE-Cy™7)
FL4 675/25 nm (eg, APC)
Optical Alignment: Fixed alignment

Fluidics
Flow Cell: 200 μm ID quartz capillary
Minimum Detectable Particle Size: 0.5 μm
Minimum Sample Volume: 50 μL
Pre-Set Flow Rates and Core Sizes
Slow : 14 μL/min, 10-μm core
Medium: 35 μL/min, 16-μm core
Fast: 66 μL/min, 22-μm core
Custom Sample Flow Rates: 10–100 μL/min
Custom Core Diameter: 5–40 μm
Recommended Sheath Fluid: 0.2-μm filtered DI water
Maximum Events Per Sample: 1 million events per well

Performance
Fluorescence Sensitivity, MESF*: FITC < 150; PE < 100
Scatter Resolution: Resolves human peripheral blood lymphocytes, monocytes, and granulocytes
Fluorescence Linearity: 2 ±0.05% for chicken erythrocyte nuclei (CEN)
Fluorescence Precision: