Jasco

JASCO J-810 FDCD (Fluorescence-Detected Circular Dichroism) Spectrometer

FDCD spectropolarimetry is a method of spectrometry to measure the difference in emission intensities that arises when an optically active sample is excited by left and right circularly polarized beams of lights. Emission perpendicular to the excitation light can be measured. The FDCD (Fluorescence detected circular dichroism) is particularly useful when measuring multichromophoric systems such as proteins, because FDCD can selectively measure the CD of a specified fluorescent chromophore. The simplest method employs the TFA-335 Total Fluorescence option utilizing high pass cut-off filters for Emission wavelength selection. This low cost method allows simple yet sensitive detection of Fluorescence changes during a titration or thermal ramp and also allows spectral Excitation scans. The FMO-427 utilizes a high efficiency monochromator for Emission wavelength selection. In addition to Excitation scans, the FMO-427 allows Emission scans to follow changes in Emission wavelength during an experiment.